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Press Info item.
06/09/2007
A new genomic marker for beef tenderness
The tenderness of beef, its flavour and its taste, depend not only on slaughter conditions and the ageing of meat, but also on the biological characteristics of muscle, which in turn are linked to the genetic traits of the animal, its rearing conditions and particularly its diet. However, the biological criteria used until now have been insufficient to explain the considerable variations observed in beef quality. INRA scientists have discovered a relationship between the expression of a gene (DNAJA1) and meat toughness. These results, which have now been patented, open the way to developing a genetic or immunological test which could identify cattle with potential to produce tender meat.
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The size and shape of a piece of beef, its colour, the amount of fat visible and the fineness of its texture are the principal criteria which, together with the purchase price, condition consumer choice. However, these characteristics do not inform as to the sensory qualities of the meat (tenderness, juiciness, flavour) after cooking. Indeed, meat quality depends on technological factors (conditions of animal slaughter, ageing, processing and cooking of the meat). But this quality also varies as a function of how the animal was reared, its genetic nature and the part of the body used which influence the biological characteristics of muscles (content in collagen, intramuscular lipids, enzymes and enzyme inhibitors, size and type of muscle fibres). However, only 30% of the variability of meat tenderness can be explained by differences in the muscle characteristics of animals. Identifying new biological characteristics of muscle with a marked influence on meat quality is thus a major challenge for the butchery industry. Particularly since these criteria could be integrated in breeding schemes for cattle.
A "tenderness" patent
In the context of a genomics research programme1, the scientists analysed the expression profiles of genes in muscles from 14 Charolais bull calves. These animals resulted from divergent breeding for muscle growth potential, considered to improve tenderness. The transcriptomes (all genes expressed) of the Longissimus thoracis muscles (fore-rib) were analysed using DNA chips and compared on the basis of sensory qualities (tenderness, juiciness, flavour) and muscle growth potential.
The researchers thus demonstrated a negative relationship between expression of the DNAJA1 gene and meat tenderness as assessed by a tasting panel. The level of expression of this gene explained up to 63% of the difference in tenderness. The Hsp 40 protein coded by this gene may therefore contribute to slowing the process of cell death (apoptosis), thus diminishing meat ageing, a process which encourages it to become more tender during the days following slaughter of the animal. Expression of this gene thus constitutes a negative marker for tenderness. This result was the subject of a patent, filed in September 2006. The scientists are now trying to validate the relationship between the expression of DNAJA1 and meat tenderness in a larger population of beef cattle (Charolais, Limousins, etc). This work should result in the development of a simple, routine test which can be used at an industrial scale to identify cattle with the potential to produce tender meat.
1This work was carried out in partnership with professionals in the cattle industry (APIS-GENE), in the context of Carine Bernard's thesis project and the MUGENE research programme under AGENAE (Analyse du GEnome des Animaux d’Elevage, or Analysis of the Genome of Livestock) funded as a result of the GENANIMAL call for tenders by the ANR.
References: Bernard C., Cassar-Malek I., Hocquette J.F., 2006. Genomic marker for meat tenderness. Patent 06 300943.5. 12 September 2006. Bernard C., Cassar-Malek I., Le Cunff M., Dubroeucq H., Renand G., Hocquette J.F., 2007. New indicators of beef sensory quality revealed by expression of specific genes. Journal of Agricultural and Food Chemistry. 55. 5229-5237.
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INRA at SPACE Rennes, September 11-14, 2007
INRA will be present at Space (Salon des Productions Animales – Carrefour Européen [Animal Production Salon – European Crossroads]), September 11-14, 2007, Parc des Expositions, Rennes-Aéroport.
On the INRA/Agrocampus Rennes stand (Hall 4 – Allée B – Stand n° 34): The genome and livestock breeding: what are the challenges for tomorrow?
A cycle of lectures: "News on agricultural research", running from Tuesday 11 to Thursday 13 septembre 2007, 14:00 to 16:00, in Salle C of the Espace Europe within SPACE. Entry free of charge.
• Tuesday September 11: The genome and livestock breeding: what challenges for the future? Madeleine Douaire, INRA-Rennes and Philippe Monget, INRA-Tours
• Wednesday September 12: Impact of biofuels on European agriculture Alexandre Gohin, INRA-Rennes Epidemiology and risk factors in livestock farming François Madec, AFSSA-Ploufragan
• Thursday September 13: Breeding factors and the quality of dairy and meat products Jean-Louis Peyraud and Jacques Mourot, INRA-Rennes
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Written by :
INRA press service, phone: +33 (0)1 42 75 91 69
Contacts :
Jean-François HOCQUETTE Tel.: 04 73 62 42 53 Jean-François Hocquette@clermont.inra.fr or Isabelle CASSAR-MALEK Tel.: 04 73 62 46 57 cassar@clermont.inra.fr
Herbivore Research Unit Animal Physiology and Livestock Systems Division Clermont-Ferrand/Theix Research Centre
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